Cell Function Reagents and Kits
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Filtered Search Results
Biotium 5-CFDA (5-Carboxyfluorescein Diacetate,
5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. Properties: Off-white solid soluble in DMSO; Store desiccated at 4°C; C25H16O9; MW: 460; [79955-27-4].
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Selleck Chemical LLC WST-8-25mg
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WST-8 is a water-soluble tetrazolium salt used for assessing cell metabolic activity that produces corresponding formazan dye that absorbs at 460 nm. WST-8 is typically used as a cell viability indicator in cell proliferation assays.
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Medchemexpress LLC FURA-2 AM 1MG
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501871915 FURA-2 AM 1MG
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Medchemexpress LLC 2-NBDG 25mg | 186689-07-6 | 342.26 | C12H14N4O8 | 25 MG
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2-NBDG is a fluorescently labeled deoxyglucose analog used to directly monitor glucose uptake in living cells and tissues. It is excitable at 488 nm and produces environment-sensitive fluorescence suitable for real-time confocal, high-resolution, or wide-field fluorescence microscopy and for flow cytometry. Supplied as a 25 mg solid with high reported purity.
- Directly reports cellular glucose uptake in live-cell assays.
- Compatible with flow cytometry and fluorescence microscopy.
- Excitable at 488 nm (argon laser).
- Environment-sensitive fluorescence for intracellular localization studies.
- High reported purity for reliable experimental results.
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Sigma Aldrich Fine Chemicals Biosciences Live Dead Cell Viability A
The Live-Dead Cell Viability Assay Kit is a quick and simple three-color assay to measure cell viability. The kit consists of Calcein-AM (stains live cells) Propidium Iodide (stains dead cells) and Hoechst 33342 (stains all cells). The kit has been optimized for 3D cell culture (spheroids human organoids and 3D matrices) and 2D cell culture (multiple cell types). The kit can be used for flow cytometry fluorescence microscopy and with fluorescence microplate readers. The kit determines viability of cells based on plasma membrane integrity and esterase activity. One kit contains reagents sufficient for 5 x 24-well plates or 12 x 96-well plates.boldAdvantages/bold 1. Tested and optimized for 3D cell culture Spheroid culture organoid culture 2D cell culture and flow cytometry.2. Easy to use and quick protocol.3. Stronger intensity of Calcein AM and Hoechst dyes compared to other products.Spectral PropertiesCalcein AM Ex/Em 490/515 nm (Green)Propidium Iodide Ex/Em 535/617 nm (Red)
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Medchemexpress LLC 2-NBDG 100mg | 186689-07-6 | 100 MG
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2-NBDG is a fluorescent D-glucose analog used to monitor glucose uptake in living cells and tissues by fluorescence-based assays. Supplied as a powder, it is typically prepared as aqueous stock solutions for cell-based experiments.
- Fluorescent glucose uptake probe suitable for live-cell assays.
- Excitation ~467 nm; emission ~542 nm.
- Supplied as a powder soluble in water and PBS.
- Typical supplier-reported purity ≥97%-98% (varies by vendor).
- Molecular weight 342.26 g/mol; CAS 186689-07-6.
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Medchemexpress LLC WST-3 | 515111-36-1 | 99.6% | 696.34 g/mol | C19H10IN6NaO10S2 | 25MG
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WST-3 is a water-soluble tetrazolium dye used as a colorimetric electron-transfer indicator in NADH-dependent assays. In the presence of an electron mediator, it is reduced by NADH to a water-soluble formazan that has an absorption maximum at 433 nm, allowing spectrophotometric quantification of reducing equivalents in cell viability and enzyme activity assays.
- Reduces to a water-soluble formazan in the presence of NADH and an electron mediator.
- Formazan product absorbs at 433 nm for spectrophotometric detection.
- High purity suitable for biochemical and cell-based assays.
- Soluble in DMSO at 100 mg/mL; aqueous formulations are achievable using co-solvents.
- Store solid protected from moisture and light at 4°C.
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Medchemexpress LLC WST-8 25MG
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501874594 WST-8 25MG
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Biotium SULFORHODAMINE 101-PEO8 SE 1MG
SULFORHODAMINE 101-PEO8 SE 1MG
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American Research Products Inc 7 AAD VIABILITY STAINING
5000265099 7 AAD VIABILITY STAINING
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Cell Signaling Technology JC-1 1 mg
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JC-1 1 mg
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US Biomax Inc CERVICAL CANCER TMA
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NC2458906 CERVICAL CANCER TMA
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Biotium Fura-2, AM Ester, 1mM In Anhydrous DMSO
Fura-2, AM ester is an intracellular fluorescent calcium indicator, and the preferred dye for ratiometric imaging microscopy. The membrane-permeant AM ester can be loaded into cells via incubation, but due to limited water solubility, a mild detergent Pluronic F-127 is often used to facilitate cell loading. Fura-2, AM ester itself does not bind Ca2, but intracellular esterases readily hydrolyze it to Fura-2. Upon calcium binding, the excitation maximum of Fura-2 undergoes a blue-shift from 363 nm (no Ca2) to 335 nm (high Ca2) while the emission maximum is relatively unchanged. Measure by flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Properties: Light yellow solid soluble in DMSO; Store at -20°C, protect from light, especially in solution; Kd: 145 nM (22°C, pH 7.2 buffer); Ex/Em = 363/512 nm, e = 27,000 M-1cm-1 (no Ca2); Ex/Em 335/505 nm, e = 35,000 M-1cm-1 (high Ca2); C44H47N3O24; MW: 1001.9; [108964-32-5].
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Biotium Furaptra, Tetrasodium Salt
Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a Kd of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca2 but with a significantly lower Kd than Fura-2 for Ca2 . An important application of Mag-Fura-2 is its use in detecting high, transient Ca2 concentration during Ca2 spikes. Mag-Fura-2/tetrasodium salt can be loaded into cells by microinjection or scrape loading. Also available are Mag-Fura-2, tetrapotassium salt and the cell membrane-permeant Mag-Fura-2 AM ester. Properties: Light yellow solid soluble in water (pH > 6); Store at 4°C and protect from light C18H10N2Na4O11; MW: 523
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Biolegend LysoFix-GBA (Lysosomal GCase Probe Yellow) Reactivity Human Apps ICC Live cell imaging Size 500 g
LysoFix-GBA (Lysosomal GCase Probe Yellow) Reactivity Human Apps ICC Live cell imaging Size 500 g
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